Equipment & Software



Beckman Coulter Cytomics FC500
Flow Cytometry Analyzer

 

Lasers
Air-cooled Argon ion laser, 488nm
Red Solid state laser, 631nm
Detectors
 Forward Scatter Side scatter detectors are photodiodes
Five photomultipliers collect the fpuorescence signalsat:
FL1 = 525 nm/40 (FITC, Alexa Fluor488, GFP, CFSE, Calcein)
FL2 = 575 nm/40 (PE)
FL3 = 620 nm/20 (PE-TexasRed, Propidium Iodide)
FL4 = 675 nm/40 (APC, PE-Cy5, Alexa Fluor 647)
FL5 = 755 nm/40 (PE-Cy7, APC-Cy7, APC-Alexa Fluor750)
Sample Preparation
Applications
Analysis Software


BD LSR II Flow Cytometer System



Lasers
Blue laser, 488nm
Red Laser, 633nm
Detectors
Forward Scatter detetctors are photodiodes
Five wave lengths and SSC detected from the 488-nm laser
530/30 nm (FITC, Alexa Fluor488, GFP, CFSE, Calcein)
575/26 nm (PE)
610/20 nm (PE-TexasRed, Propidium Iodide)
695-40 nm (PE-Cy5.5, PERCP-Cy5.5)
780/60 nm (PE-Cy7)
Three Wave lengths from the 633-nm laser
660/20 nm (APC)
710/50 nm (Alexa Fluor 700)
780/90 nm (APC-Cy7)
Sample Preparation
Applications
Analysis Software


Cytopeia InFlux cell sorter
Fluorescence activated cell sorting (FACS) separates the cells in a suspension on the basis of size and the color of their fluorescence. Currently the InFlux facilitates two-way sorting. Sorted cells can be collected in tubes or 96-well plates.


Lasers
Air-cooled Argon ion laser, 488nm
Air-cooled Helium - Neon ion laser, 633nm
The third laser can be added in the future if necessary.
Detectors
Forward Scatter and Side Scatter detectors
Photomultipliers collect the fluorescence signals at:
531/40 nm, 572-27nm, 610/20 nm, 670/40 nm, >75 nm
Sample Preparation
Appllications




Veritas Laser Capture Microdissection (LCM) and Laser Cutting System from Molecular Devices.


Laser Capture Microdissection is a new technology for isolating individual cells or groups of cells from specific microscopic regions of tissue sections. The cells of interest can be collected for genomic and proteomic analysis.

If you are interested in using this technology to obtain cells or groups of cells from frozen tissue sections, Please contact Xiangle Sun at 817-735-0117 for further information.
You will only be charged for the materials used. Successful studies have been done in mouse lung and rat ocular tissues in this core facility. Useful websites:
NIH Laser Capture Microdissection
Laser Capture Microdissection


 

 

Olympus AX70 upright microscope with fluorescence and phase contrast optics for immunofluorescence Imaging.




The Fluorescence Microscope is equipped with
Olympus UIS2 Objectives: 4X/0.16, 10X/0.3, 20X/0.5/ 40X/0.75 and 100X/ oil.
3 fluorescence filters with the new hard surface coatings that are extremely high contrast and virtually eliminate bleedthrough can be utilized.
DAPI(blue) Semrock DAPI-1160A
(Optimized for these fluorophores: DAPI,Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP)
FITC (green) Semrock FITC-3540B
(Optimized for these fluorophores: FITC, CFSE, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, Mito Tracker Green, Oregon Green, Rhodamine 110, rsGFP 9red-shifted GFP, S65T)
Texas-Red (red) Semrock TXRED-4040B
(Optimized for these fluorophores: Texas-Red, 5-ROX, Alexa Fluor 568&594, Calcium Crimson, Cy3.5, HcRed, and Mito Tracker Red.)
Olympus DP 70 Digital Camera for high resolution imaging.
Image-Pro Plus 5.1 Image analysis Software