Laszlo Prokai , Ph.D., D.Sc.
Professor and Robert A. Welch Chair in Biochemistry
Department: Pharmacology & Neuroscience
Office: RES 466
Phone: 817-735-2206
Fax: 817-735-2118
Email: Laszlo.Prokai@unthsc.edu
Faculty Homepage

 

Biographical Sketch | Education | Publications | Patents | Awards and Honors

Biographical Sketch

Dr. Prokai started his academic career as an Assistant Professor at the Department of Pharmaceutics and the Center for Drug Discovery of the College of Pharmacy at the University of Florida in 1991. During his tenure there, he rose in rank to Professor in the Department of Medicinal Chemistry, held an Affiliate Professorship at the Department of Chemistry, was a Joint Professor of the Department of Anesthesiology, Scientific Advisor of the Biotechnology Program, and Member of the McKnight Brain Institute. Dr. Prokai joined the University of North Texas Health Science Center in 2005. He is currently Professor and the Robert A. Welch Chair in Biochemistry. Dr. Prokai also is Courtesy Professor at the Department of Medicinal Chemistry, University of Florida, and Adjunct Professor at the Department of Chemistry and Biochemistry, University of Texas at Arlington and at the Department of Chemistry of the Texas Christian University. Dr. Prokai has maintained an actively funded research program supported by the National Institutes of Health (NIH) and is recognized nationally and internationally for his work on discovery, bioorganic and medicinal chemistry of central nervous system agents, as well as on neuropeptides, proteomics and mass spectrometry. Dr. Prokai has been an invited speaker on numerous national and international scientific meetings, received a Pro Renovanda Cultura Hungariae Visiting Professorship at the Department of Medical Chemistry, University of Szeged (Hungary) in 2002, and has been an active collaborator with pharmaceutical and chemical companies. He currently serves on the Editorial Boards of Current Medicinal Chemistry, Central Nervous System Agents in Medicinal Chemistry, Current Drug Delivery, Medicinal Chemistry Reviews-Online, Medicinal Chemistry, Journal of Data Mining in Genomics & Proteomics, and the Journal of Integrated Omics.

Research Interests
Dr. Prokai's current research focuses on
(1) Novel therapies against neurodegenerative and ophthalmic diseases using site-selective prodrugs.
(2) Development and use of proteomics in aging research, studying neurodegenerative diseases and cancer, with especial attention to quantitative expression profiling and oxidative stress-associated posttranslational modifications.
(3) Discovering new therapeutic agents based on neuropeptides and peptidomimetics as lead molecules.
These multidisciplinary projects rely on his broad technical expertise in state-of-the-art methods in bioorganic, medicinal and bioanalytical chemistry, and on excellent facilities featuring four mass spectrometers.

Education

B.Sc. 1978 Chemical Engineering; Summa Cum Laude, University of Veszprem (now University of Pannonia), Veszprem,Hungary
M.Sc. 1980 Chemical Engineering (Radiochemical Technology); Summa Cum Laude, University of Veszprem (now University of Pannonia), Veszprem, Hungary
Ph.D. 1983 Radiochemistry; Summa Cum Laude, University of Veszprem (now University of Pannonia), Veszprem, Hungary
Postdoctoral training in Medicinal Chemistry, 1987 to 1989; Department of Medicinal Chemistry, University of Florida, Gainesville, FL

Publications

Monograph
Prokai L. Field Desorption Mass Spectrometry, Practical Spectroscopy Monograph Series, Marcel Dekker, New York, 1990, pp. 304.

Book Edited
Prokai L, Prokai-Tatrai K. Peptide Transport and Delivery Into the Central Nervous System, Progress in Drug Research, Vol. 61, Birkhäuser, Basel, 2003, pp. 245.

Selected Book Chapters (Recent)
Rauniyar N, Prokai L. Mass spectrometry-based methods to investigate posttranslational protein modifications by lipid-peroxidation products, in Handbook of Mass Spectrometry (M. Lee, Ed.), John Wiley & Sons, Chichester, UK, 2012, 23-40.

Prokai-Tatrai K, Prokai L. Prodrugs for brain delivery of small neuropeptides, Chapter 21 in Neuropeptides: Methods and Protocols (A. Merighi, Ed.), Methods in Molecular Biology, Vol. 789, Humana–Springer, Totowa, NJ, 2011, 313-336.

Prokai L. Application of mass spectrometry in brain research: neurotransmitters, neuropeptides, and brain proteins, in Medical Applications of Mass Spectrometry (K. Vekey, A. Telekes, A. Vertes, Eds.), Elsevier, Amsterdam, 2008, 407-424.

Prokai-Tatrai K, Prokai L. The impact of metabolism on the safety of estrogen therapy, in the Annals of the New York Academy of Sciences, Vol. 1052 (M. Singh, Ed.), New York Academy of Sciences, New York, NY, 2005, 243-257.

Selected Peer-Reviewed Papers (Recent)
Szarka S, Prokai-Tatrai K, Prokai L. Application of screening experimental designs to assess chromatographic isotope effect upon isotope-coded derivatization for quantitative liquid chromatography–mass spectrometry. Anal Chem. 2014 July;86(14):7033-40.

Prokai L, Guo J, Prokai-Tatrai K. Selective chemoprecipitation to enrich nitropeptides from complex proteomes for mass spectrometric analysis. Nat Protocols. 2014 April;9(4):882-95.

Talamantes T, Ughy B, Domonkos I, Kis M, Gombos Z, Prokai L. Label-free LC–MS/MS identification of phosphatidylglycerol-regulated proteins in Synechocystis sp. PCC6803. Proteomics. 2014 April;14(9):1053-7.

Prokai D, Nguyen T, Kamrowski K, Chandra A, Talamantes T, Baxter LR Jr, Prokai L. An exploratory evaluation of tyrosine hydroxylase inhibition in planaria as a model for parkinsonism. Int J Mol Sci. 2013 December;14(12):23289-96.

Stein GE, Throckmorton J, Sharmen A, Weiss WJ, Prokai L, Smith C, Havlichek D. Tissue penetration and antimicrobial activity of standard and high-dose trimethoprim/sulfamethoxazole and linezolid in serum from patients with diabetic foot infection. J Antimicrobiol Chemother. 2013 December;68(12):2852-58.

Nagaprashantha LD, Talamantes T, Singhal J, Guo J, Vatsyayan R, Rauniyar N, Awasthi S, Singhal SS, Prokai L. Proteomic analysis of signaling network regulation in renal cell carcinomas with differential hypoxia-inducible factor-2α expression. PLoS One. 2013 August;8(8):e71654.

Prokai-Tatrai K, Hua X, Nguyen V, Szarka S, Prokai L, Koulen P. 17β-Estradiol eye drops protect the retinal ganglion cell layer and preserve visual function in an in vivo model of glaucoma. Mol Pharmaceutics. 2013 August;10(8):3253–61.

Guo J, Prokai-Tatrai K, Prokai L. Differential quantitation of protein nitration by liquid chromatography-mass spectrometry using isotope-coded dimethyl labeling and chemoprecipitation. J Chromatogr A. 2012 April;1232(1):266-75.

Rauniyar N, Prokai L. Isotope-coded dimethyl tagging for differential quantification of posttranslational protein carbonylation by 4-hydroxy-2-nonenal, an end-product of lipid peroxidation. J Mass Spectrom. 2011 October;46(10):976-85.

Guo J, Prokai-Tatrai K, Nguyen V, Rauniyar N, Ughy B, Prokai L. Protein targets for carbonylation by 4-hydroxy-2-nonenal in rat liver mitochondria. J Proteomics. 2011 October;74(11):2370-9.

Prokai-Tatrai K, Guo J, Prokai L. Selective chemoprecipitation and subsequent release of tagged species for the analysis of nitropeptides by liquid chromatography–tandem mass spectrometry. Mol Cell Proteomics. 2011 August;10(10):10.1074/mcp.M110.002923,1-10

Nagaprashantha LD, Vatsyayan R, Singhal J, Lelsani P, Prokai L, Awasthi S, Singhal SS. 2’-Hydroxyflavanone inhibits proliferation, tumor vascularization and promotes normal differentiation in VHL-mutant renal cell carcinoma. Carcinogenesis. 2011 April;32(4):568-575.

Laczko-Dobos H, Frycak P, Ughy B, Domonkos I, Wada H, Prokai L, Gombos Z. Remodeling of phosphatidylglycerol in Synechocystis PCC6803. Biochim Biophys Acta - Mol Cell Biol Lipids. 2010 February; 1801(2):163-70.

Prokai L, Stevens SM, Rauniyar N, Nguyen V. Rapid label-free identification of estrogen-induced differential protein expression in vivo from mouse brain and uterine tissue. J Proteome Res. 2009 August; 8(8):3862-71.

Prokai-Tatrai K, Prokai L. Prodrugs of thyrotropin-releasing hormone and related peptides as central nervous system agents. Molecules. 2009 February; 14(2):633-54.

Rauniyar N, Stevens SM, Prokai-Tatrai K, Prokai L. Characterization of 4-hydroxy-2-nonenal-modified peptides by liquid chromatography-tandem mass spectrometry using data-dependent acquisition: neutral loss-driven MS3 versus neutral loss-driven electron capture dissociation. Anal Chem. 2009 Jan;81(2):782-9.

Stevens SM, Prokai-Tatrai K, Prokai L. Factors that contribute to the misidentification of tyrosine nitration by shotgun proteomics. Mol Cell Proteomics. 2008 Dec;7(12):2442-51.

Stevens SM, Duncan RS, Koulen P, Prokai L. Proteomic analysis of mouse brain microsomes: identification and bioinformatic characterization of endoplasmic reticulum proteins in the mammalian central nervous system. J Proteome Res. 2008 March;7(3):1046-54.

Prokai-Tatrai K, Perjesi P, Rivera-Portalatin NM, Simpkins JW, Prokai L. Mechanistic investigations on the antioxidant action of a neuroprotective estrogen derivative. Steroids. 2008 March;73(3):280-8.

Branca RMM, Bodo G, Bagyinka C, Prokai L. De novo sequencing of a 21-kDa cytochrome c4 from Thiocapsa roseopersicina by nanoelectrospray ionization ion-trap and Fourier-transform ion-cyclotron resonance mass spectrometry. J Mass Spectrom. 2007 Dec;42(12):1569-82.

Rivera-Portalatin NM, Serrano-Vera JL, Prokai-Tatrai K, Prokai L. Comparison of estrogen-derived ortho-quinone and para-quinol concerning induction of oxidative stress. J Steroid Biochem Mol Biol. 2007 June-July;105(1-5):71-5.

Prokai-Tatrai K, Rivera-Portalatin N, Rauniyar N, Prokai L. A facile microwave-assisted synthesis of p-quinols by lead(IV)acetate oxidation. Lett Org Chem. 2007 June;4(4):265-7.

Prokai L, Simpkins JW. Structure-nongenomic neuroprotection relationship of estrogens and estrogen-derived compounds. Pharmacol Therapeut. 2007 April;114(1):1-12.

Prokai L, Zharikova AD, Juhasz A, Prokai-Tatrai K. Cardiovascular effects of neuropeptide FF antagonists. Peptides. 2006 May;27(5):1015-9.

Prokai L, Prokai-Tatrai K, Perjesi P, Simpkins JW. Mechanistic insights into the direct antioxidant effects of estrogens. Drug Dev Res. 2005 Oct;66(6):118-25.

Stevens SM, Chung AY, Chow MC, McClung SH, Strachan CN, Harmon AC, Denslow ND, Prokai L. Enhancement of phosphoprotein analysis using a fluorescent affinity tag and mass spectrometry. Rapid Commun Mass Spectrom. 2005 Jun;19(15):2157-62.

Bergeron RJ, Bharti N, Wiegand J, McManis JS, Yao H, Prokai L. Polyamine-vectored iron chelators: the role of charge. J Med Chem. 2005 Jun;48(12):4120-37.

Simpkins JW, Wang J, Wang X, Perez E, Prokai L, Dykens JA. Mitochondria play a central role in estrogen-induced neuroprotection. Curr Drug Targets CNS Neurol Disord. 2005 Feb;4(1):69-83.

Prokai L, Zharikova AD, Stevens SM. Effect of chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on isotope-coded affinity tags and liquid chromatography/mass spectrometry. J Mass Spectrom. 2005 Feb;40(2):169-75.

Stevens Jr SM, Prokai-Tatrai K, Prokai L. Screening of combinatorial libraries for substrate preference by mass spectrometry. Anal Chem. 2005 Jan;77(2):698-701.

Prokai L, Prokai-Tatrai K, Zharikova AD, Nguyen V, Perjesi P, Stevens SM. Centrally acting and metabolically stable thyrotropin-releasing hormone analogues by replacement of histidine with substituted pyridinium. J Med Chem. 2004 Nov;47(24):6025-33.

Yang SH, Liu R, Perez EJ, Wen Y, Stevens SM, Valencia T, Brun-Zinkernagel AM, Prokai L, Will Y, Dykens J, Koulen P, Simpkins JW. Mitochondrial localization of estrogen receptor beta. Proc Natl Acad Sci U S A. 2004 Mar;101(12):4130-5.

Ross BP, Braddy AC, McGeary RP, Blanchfield JT, Prokai L, Toth I. Micellar aggregation and membrane partitioning of bile salts, fatty acids, sodium dodecyl sulfate, and sugar-conjugated fatty acids: correlation with hemolytic potency and implications for drug delivery. Mol Pharmaceutics. 2004 Jan;1(3):233-45.

Kem WR, Mahnir VM, Prokai L, Papke RL, Cao X, LeFrancois S, Wildeboer K, Prokai-Tatrai K, Porter-Papke J, Soti F. Hydroxy metabolites of the Alzheimer's drug candidate 3-[(2,4-dimethoxy)benzylidene]-anabaseine dihydrochloride GTS-21): their molecular properties, interactions with brain nicotinic receptors, and brain penetration. Mol Pharmacol. 2004 Jan;65(1):56-67.

Prokai L, Prokai-Tatrai K, Perjesi P, Zharikova D, Perez J, Liu R, Simpkins JW. Quinol-based cyclic antioxidant mechanism in estrogen neuroprotection. Proc Natl Acad Sci U S A. 2003 Sep;100(20):11741-6.

Patents
Antagonists of RF-Amide Neuropeptides, US Patent 6,797,707
Antagonists of RF-Amide Neuropeptides, US Patent 6,960,574
Steroidal Quinols and Their Use for Antioxidant Therapy, US Patent 7,026,306
Thyrotropin-releasing hormone analogues and their therapeutic applications, US Patent 7,067,257
Prodrugs for Use as Ophthalmic Agents, US Patent 7,186,707
Steroidal Quinols and Their Use for Estrogen Replacement Therapy, US Patent 7,300,926
Quinol Compound and Pharmaceutical Composition, US Patent 7,534,779
Prodrugs for Use as Ophthalmic Agents, US Patent 7,572,781
HDL Particles for Delivery of Nucleic Acids, US Patent 8,734,853

Awards and Honors
Candidate of Science in Chemistry (C.Sci), by the Hungarian Academy of Sciences in 1987
Habilitation (Dr. habil.) by the University of Veszprem in 2000
Leading Scientist of the World 2006, International Biographical Centre, Cambridge, England
Doctor Academiae Scientiarum Hungaricae (D.Sc.) by the Hungarian Academy of Sciences in 2010