Mass Spectrometry and Proteomics Laboratory

Our laboratory is located at the Center for Neuroscience Discovery of the Institute for Healthy Aging at the UNTHSC. It is a research-oriented laboratory; however, we offer sample analysis, data processing and interpretation by laboratory personnel for researchers in the UNTHSC and also to external academic institutions and industry. Prior arrangement with the Director of the Laboratory is required.

We have two LC-MS systems and a GC-MS (from Thermo Scientific, San Jose, CA). Software resources for proteomics and bioinformatics include Proteome Discoverer, Sequest, Mascot, Scaffold, perSPECtives, Ingenuity Pathways Analyses, and many more.

Routine mass spectrometry and proteomic services include: MS molecular weight determination (small molecules and large molecules <100,000 Da), LC-MS, GC-MS, tandem mass spectrometry (MS/MS, MSn), protein identification by proteolytic digestion, nano LC-MS/MS and database search, quantitative proteomics. Please contact Laszlo.Prokai@unthsc.edu to discuss custom work such as complex proteomic analysis.

Thermo Scientific linear ion-trap / Orbitrap hybrid instrument (high-resolution MS and MSn), coupled with a EASY nLC 1000 system that uses Microfluidic Flow Control to generate precise gradients for liquid chromatography (LC) at nanoscale flow rate. This increases mass spectrometer sensitivity and improves identification of low-abundance proteins. A regular-flow HPLC system (Agilent 1100) is also available. The system provides high resolution and sensitivity coupled with <3 ppm/sub-ppm mass accuracy. Available methods of ionization are electrospray (both regular and nanoflow), atmospheric-pressure chemical ionization (APCI), atmospheric-pressure photoionization (APPI) and direct analysis in real time (DART). Besides collision-induced dissociation (CID) and higher-energy CID (HCD), electron transfer dissociation (ETD) can also be applied. Triversa NanoMate, a chip-based infusion system, is installed for advanced/high throughput ESI-MS (including MS/MS and MSn) and combined on-line/off-line LC-MS.
TSQ Quantum Ultra (Triple-stage quadrupole tandem mass spectrometer): Available methods of ionization are electrospray (both regular and high-temperature, HESI), atmospheric-pressure chemical ionization (APCI), atmospheric-pressure photoionization (APPI) and direct analysis in real time (DART). High-performance liquid chromatographs (Surveyor MS and Eldex MicroPro) are connected to the TSQ Quantum Ultra, which permits separation at normal flow (0.2-2.0 mL/min, Surveyor) and microflow (5-20 μL/min, Eldex).
PolarisQ (Quadrupole ion trap, GC/MS, EI/CI, MS/MS).This system can be used for separation of complex mixtures by gas chromatography and identification by mass spectrometry. Available methods of ionization: electron ionization (EI) and chemical ionization (CI).