Advanced Mass Spectrometry and Proteomics Laboratory
HOME RATES SERVICE STAFF SAMPLE SUBMISSION PUBLICATIONS
Mass Spectrometry - Protein
Characterization & Proteomics
Expression Profiling and Proteome
Characterization
Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic. Characterization of Endoplasmic Reticulum Proteins in the
Mammalian Central Nervous System.
J Proteome Res. 2008 March;7(3):1046-54. Stevens SM Jr, Duncan RS, Koulen P, Prokai L.
-Ref 2:
Effect of chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on
isotope-coded affinity tags and liquid chromatography/mass spectrometry.
J Mass Spectrom. 2005 Feb;40(2):169-75. Prokai L, Zharikova AD, Stevens SM Jr.
-Ref 3:
Proteomic analysis of the synaptic plasma membrane fraction isolated from rat forebrain.
Brain Res Mol Brain Res. 2003 Oct 7;117(2):116-28. Stevens SM Jr, Zharikova AD, Prokai L.
Posttranslational
Modifications
-Ref 1:
Mass spectrometry-based survey of age-associated protein carbonylation in rat brain mitochondria.
J Mass Spectrom. 2007 Dec;42(12):1583-9. J Mass Spectrom. 2007 Dec;42(12):1583-9. Prokai L, Yan LJ, Vera-Serrano JL, Stevens SM, Forster MJ.
-Ref 2:
Fourier transform ion cyclotron resonance mass spectrometry of covalent adducts of proteins and 4-hydroxy-2-nonenal, a reactive end-product
of lipid peroxidation.
J Mass Spectrom. 2007 Dec;42(12):1569-82. Rauniyar N, Stevens SM Jr, Prokai L.
-Ref 3:
Rapid characterization of covalent modifications to rat brain mitochondrial proteins after ex vivo exposure to 4-hydroxy-2-nonenal by liquid
chromatography-tandem mass spectrometry using data-dependent and neutral loss-driven MS^3 acquisition.
J Mass Spectrom. 2007 Dec;42(12):1599-605. Stevens SM Jr, Rauniyar N, Prokai L.
De Novo Protein
Sequencing
-Ref 1:
De novo sequencing of a 21-kDa cytochrome c4 from Thiocapsa roseopersicina by nanoelectrospray ionization ion-trap and Fourier-transform
ion-cyclotron resonance mass spectrometry.
J Mass Spectrom. 2007 Dec;42(12):1569-82. Branca RM, Bodó G, Bagyinka C, Prokai L.
LC/MS: Pharmacokinetics & Drug Distribution
-Ref 1:
Centrally acting and metabolically stable thyrotropin-releasing hormone analogues by replacement of histidine with substituted pyridinium.
J Med Chem. 2004 Nov 18;47(24):6025-33. Prokai L, Prokai-Tatrai K, Zharikova AD, Nguyen V, Perjesi P, Stevens SM Jr.
-Ref 2:
Integration of mass spectrometry into early-phase discovery and development of central nervous system agents.
J Mass Spectrom. 2001 Nov;36(11):1211-9. Prokai L, Zharikova AD, Janáky T, Li X, Braddy AC, Perjesi P, Matveeva L, Powell DH, Prokai-Tatrai K.
-Ref 3:
Exploratory pharmacokinetics and brain distribution study of a neuropeptide FF antagonist by liquid chromatography/atmospheric pressure
ionization tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2000;14(24):2412-8. Prokai L, Zharikova AD, Janáky T, Prokai-Tatrai K.
Contact
information:
Department of Molecular Biology and Immunology
University of North
Texas Health Science Center
3500 Camp Bowie Blvd
Ft. Worth, TX 76107
Tel: 817.735.0619,
817.735.0629, Fax: 817.735.2118
E-mail: mkukula@hsc.unt.edu
Webpage:http://www.hsc.unt.edu/prokai/