Expression Profiling and Proteome Characterization

Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic. Characterization of Endoplasmic Reticulum Proteins in the Mammalian Central Nervous System. J Proteome Res.2008 March;7(3):1046-54.   Stevens SM Jr, Duncan RS, Koulen P, Prokai L.  

Effect of  chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on isotope-coded affinity tags and        liquid chromatography/mass spectrometry. J Mass Spectrom.2005 Feb;40(2):169-75.   Prokai L, Zharikova AD, Stevens SM Jr.

Proteomic analysis of the synaptic plasma membrane fraction isolated from rat forebrain. Brain Res Mol Brain Res.2003 Oct 7;117(2):116-28.  Stevens SM Jr, Zharikova AD, Prokai L.

Posttranslational Modifications

Characterization of 4-hydroxy-2-nonenal-modified peptides by liquid chromatography-tandem mass spectrometry using data-dependent acquisition: neutral loss-driven MS³   versus neutral loss-driven electron capture dissociation. Anal Chem.2009 Jan;81(2):782-9.  Rauniyar N, Stevens SM, Prokai-Tatrai K, Prokai L.

Factors that contribute to the misidentification of tyrosine nitration by shotgun proteomics. Mol Cell Proteomics.2008 Dec;7(12):2442-51.  Stevens SM, Prokai-Tatrai K, Prokai L.

Mass spectrometry-based survey of age-associated protein carbonylation in rat brain mitochondria. J Mass Spectrom.2007 Dec;42(12):1583-9.   Prokai L, Yan LJ, Vera-Serrano JL, Stevens SM, Forster MJ.

Fourier transform ion cyclotron resonance mass spectrometry of covalent adducts of proteins and 4-hydroxy-2-nonenal, a reactive end-product of lipid peroxidation. Anal Bioanal Chem.2007 Nov;389(5):1421-8.  Rauniyar N, Stevens SM Jr, Prokai L.

Rapid characterization of covalent modifications to rat brain mitochondrial proteins after ex vivo exposure to 4-hydroxy-2-nonenal by liquid chromatography-tandem mass spectrometry using data-dependent and neutral loss-driven MS^3 acquisition.  J Mass Spectrom.2007 Dec;42(12):1599-605.  Stevens SM Jr, Rauniyar N, Prokai L.

De novo sequencing of a 21-kDa cytochrome c4 from Thiocapsa roseopersicina bynanoelectrospray ionization ion-trap and Fourier-transformion-cyclotronresonance mass spectrometry. J Mass Spectrom.2007 Dec;42(12):1569-82. Branca RM, Bodó G, Bagyinka C, Prokai L.