Advanced Mass Spectrometry and Proteomics Laboratory

Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic. Characterization of Endoplasmic Reticulum Proteins in the 

Mammalian Central Nervous System. 

J Proteome Res. 2008 March;7(3):1046-54. Stevens SM Jr, Duncan RS, Koulen P, Prokai L.

-Ref 2: 

Effect of chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on 

isotope-coded affinity tags and liquid chromatography/mass spectrometry. 

J Mass Spectrom. 2005 Feb;40(2):169-75. Prokai L, Zharikova AD, Stevens SM Jr. 

-Ref 3: 

Proteomic analysis of the synaptic plasma membrane fraction isolated from rat forebrain.

Brain Res Mol Brain Res. 2003 Oct 7;117(2):116-28. Stevens SM Jr, Zharikova AD, Prokai L.

Posttranslational Modifications

-Ref 1:

Mass spectrometry-based survey of age-associated protein carbonylation in rat brain mitochondria.

J Mass Spectrom. 2007 Dec;42(12):1583-9. J Mass Spectrom. 2007 Dec;42(12):1583-9. Prokai L, Yan LJ, Vera-Serrano JL, Stevens SM, Forster MJ.

-Ref 2:

Fourier transform ion cyclotron resonance mass spectrometry of covalent adducts of proteins and 4-hydroxy-2-nonenal, a reactive end-product

of lipid peroxidation. 

J Mass Spectrom. 2007 Dec;42(12):1569-82. Rauniyar N, Stevens SM Jr, Prokai L. 

-Ref 3:

Rapid characterization of covalent modifications to rat brain mitochondrial proteins after ex vivo exposure to 4-hydroxy-2-nonenal by liquid

chromatography-tandem mass spectrometry using data-dependent and neutral loss-driven MS^3 acquisition. 

J Mass Spectrom. 2007 Dec;42(12):1599-605. Stevens SM Jr, Rauniyar N, Prokai L. 

De Novo Protein Sequencing

-Ref 1:

De novo sequencing of a 21-kDa cytochrome c4 from Thiocapsa roseopersicina by nanoelectrospray ionization ion-trap and Fourier-transform 

ion-cyclotron resonance mass spectrometry. 

J Mass Spectrom. 2007 Dec;42(12):1569-82. Branca RM, Bodó G, Bagyinka C, Prokai L. 

LC/MS: Pharmacokinetics & Drug Distribution

-Ref 1:

Centrally acting and metabolically stable thyrotropin-releasing hormone analogues by replacement of histidine with substituted pyridinium.

J Med Chem. 2004 Nov 18;47(24):6025-33. Prokai L, Prokai-Tatrai K, Zharikova AD, Nguyen V, Perjesi P, Stevens SM Jr. 

-Ref 2:

Integration of mass spectrometry into early-phase discovery and development of central nervous system agents.

J Mass Spectrom. 2001 Nov;36(11):1211-9. Prokai L, Zharikova AD, Janáky T, Li X, Braddy AC, Perjesi P, Matveeva L, Powell DH, Prokai-Tatrai K.

Exploratory pharmacokinetics and brain distribution study of a neuropeptide FF antagonist by liquid chromatography/atmospheric pressure 

ionization tandem mass spectrometry. 

Rapid Commun Mass Spectrom. 2000;14(24):2412-8. Prokai L, Zharikova AD, Janáky T, Prokai-Tatrai K.

Contact information:
Department of Molecular Biology and Immunology
University of North Texas Health Science Center  
3500 Camp Bowie Blvd
Ft. Worth, TX 76107
Tel: 817.735.0619,   817.735.0629,   Fax: 817.735.2118
E-mail: mkukula@hsc.unt.edu       Webpage:http://www.hsc.unt.edu/prokai/