Publications
The biology of the (peri)menopause/hormone therapy

Yang SH, Liu R, Wen Y, Perez E, Cutright J, Brun-Zinkernagel AM, Singh M, Day AL, Simpkins JW. 2005 A Neuroendocrine Mechanism for tolerance to Cerebral Ischemia-Reperfusion Injury in Male Rats.  J. Neurobiology 62:341-51.

Singh M and Simpkins JW 2004 for the CARPE group. Consortium for the Assessment of Research on Progestins and Estrogens (CARPE), Womens Health (Larchmt). 10:1165-8

Simpkins JW, Yang SH, Wen Y, Singh M. 2005. Estrogens, progestins, menopause and neurodegeneration: basic and clinical studies. Cell. Mol. Life Sci. 62: 271–280

Singh M. 2005 Book Review: The Neuroendocrine Immune Network in Ageing Volume Editors: Rainer H. Straub and Eugenio Mocchegiani, Volume 4 of Neuroimmune Biology Elsevier (2004)441. ISBN 0-444-51617-4. Experimental Gerontology 40: 255–256

Singh M. 2005 Mechanisms of Progesterone-induced Neuroprotection, in The Future of Estrogen and Hormone Therapy in Postmenopausal Women: What Basic Science and Clinical Studies Teach Us. Singh M and Simpkins JW (Eds). Annals of the New York Academy of Science 2005 Vol. 1052

Sétáló Jr. G, Singh M, Nethrapalli IS and Toran-Allerand CD. 2005 Protein Kinase C Activity is Necessary for Estrogen-induced ERK Phosphorylation in Neocortical Explants. Neurochemical Research 30:779-90.

The Future of Estrogen and Hormone Therapy in Postmenopausal Women: What Basic Science and Clinical Studies Teach Us. 2005. Singh M and Simpkins JW (Eds). Annals of the New York Academy of Science Vol 1052)

Gatson JG, Kaur P, Singh M. 2005 Dihydrotestosterone Differentially Modulates the MAPK and the PI-3 Kinase/Akt Pathways Through the Nuclear and Novel Membrane Androgen Receptor in C6 Cells. Endocrinology 147(4):2028-2034.

Singh M, Dykens JA and Simpkins JW. 2006 Estrogens as Neuroprotectant Agents: Mechanisms and Clinical Implications. Experimental Biology and Medicine (Maywood) 231(5):514-21.

Singh M. 2006 Progesterone-induced Neuroprotection. Endocrine 29(2):271-74

Bell Horner CL, Dohi A, Nguyen RQ, Dillon GH, Singh, M. 2006 The ERK/MAPK pathway regulates GABAA receptors. J. Neurobiol (in press)

Shao-Hua Yang, Ran Liu, Samuel S. Wu, James W. Simpkins, The Use of Estrogens and Related Compounds in the Treatment of Damage from Cerebral Ischemia, In Steroids and the Nervous System, Annuals of the New York Academy of Sciences 1007: 101-107, 2004.

Cammarata, P. R., S. Chu, A. Moor, S.-H. Yang and J. W. Simpkins, Subcellular distribution of native estrogen receptors α and β in cultured human lens epithelial cells, Investigational Eye Research 78: 861-871, 2004.

Yang, S. H., J. Wang and J. W. Simpkins, Novel strategies for neuroprotection against Acute Brain Damage, in A Textbook on Neurointensive Care, Eds. A. J. Layon, A. Gabrielle and W. A. Friedman, W. B. Saunders Company, pp 849-863, 2004.

Shao-Hua Yang1*; Ran Liu1; Stanley M. Stevens, Jr.2; Thomas Valencia3, Yi Wen1, Anne-Marie Brun-Zinkernagel4; Laszlo Prokai2; Yvonne Will5, James Dykens5; Peter Koulen1, James W. Simpkins 1 Mitochondrial localization of estrogen receptor beta, Proc. National Academy of Sciences USA, 101: 4130-4135, 2004.

Wen, Y., S.-H. Yang, R. Liu, E. J.  Perez, K. D. Yi, P. Koulen and J. W. Simpkins, Estrogen attenuates post-ischemic and nuclear factor-kappa B activation induced by transient cerebral ischemia, Brain Research 1008: 147-154 , 2004.

Wen, Y., E. J. Perez, P.S. Green and J.W. Simpkins, nNOS mediates neuroprotective effects of 17 b-estradiol in neuroblastoma cells, NeuroReport  15: 1515-1518, 2004.

James W. Simpkins, Jian Wang, X. Wang, Evelyn Perez, Laszlo Prokai and James A. Dykens, Mitochondria Play a Central Role in Estrogen-Induced Neuroprotection, Current Drug Targets-CNS and Neurological Disorders, 4:69-83  2005.

Simpkins, J. W., S.H. Yang, Y. Wen and M. Singh, Neuroprotective Effects of Female Sex Steroids: Animal Studies, Cellular and Molecular Life Science, in press, 2004.

Jung, M. E, M. Rewal, E. Perez and J.W. Simpkins, Estrogen protects against brain lipid peroxidation in ethanol withdrawn rats. Pharmacology, Biochemistry & Behavior 79: 573-586, 2004.
Rewal, M. M.E. Jung and James W. Simpkins, Role of the GABA-A system in estrogen-induced protection against brain lipid peroxidation in ethanol-withdrawn rats, Alcohol: Clinical and Experimental Research, Alcohol Clin Exp Res. 28:1907-1915, 2004.

Simpkins J. W. and M. Singh for the CAPRE Group, Consortium for the Assessment of Research on Progestins and Estrogens (CARPE), J. Women’s Health 13: 1165-1168, 2004.

Simpkins, J.W., S.H. Yang, R. Liu, E. Perez, Z.Y.Cai, D.F. Covey and P.S. Green, estrogen-like compounds for ischemic neuroprotection. Stroke Stroke 35 (Suppl. 1) 2648-2651, 2004.
Shao-Hua Yang, Ran Liu, Samuel S. Wu, James W. Simpkins, The Use of Estrogens and Related Compounds in the Treatment of Damage from Cerebral Ischemia, In Steroids and the Nervous System, Annuals of the New York Academy of Sciences 1007: 101-108, 2004.

Yang, S.H., R. Liu, Y. Wen, E. Perez, J. Cutright, A.M Brun-Zinkernagel, M.Singh, A.L. Day and J. W. Simpkins, Neuroendocrine mechanism for tolerance to cerebral ischemia-reperfusion injury in male rats, J. Neurobiology 62: 341-351, 2004.

James W. Simpkins, Jian Wang, X. Wang, Evelyn Perez, Laszlo Prokai and James A. Dykens, Mitochondria Play a Central Role in Estrogen-Induced Neuroprotection, Current Drug Targets-CNS and Neurological Disorders, 4:69-83, 2005.

Jung, M. E., M. B. Gatch and J. W. Simpkins, Estrogen Neuroprotection Against the Neurotoxic Effects of Ethanol Withdrawal: Potential Mechanisms, Experimental Biology & Medicine 230: 8-22, 2005.

James W. Simpkins, ShaoHua Yang, and Yi Wen, Use of Estrogens as Neuroprotectants in Stroke and Alzheimer’s Disease, In Synaptic Plasticity: From Basic Mechanisms to Clinical Application, Eds. M. Baudry, A. Bi and S. S. Schreiber, Marcel Dekker, Inc., New York, pp 175-188, 2005.
Simpkins, J. W., S.H. Yang, Y. Wen and M. Singh, Estrogens, progestins, menopause and neurodegeneration: basic and clinical studies, Cellular and Molecular Life Science 62: 271-280, 2005.

Rewal, M., Y. Wen, J.W. Simpkins and M.E. Jung, Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens, Neuroscience Letters 377: 44-48, 2005.

Kumar, D. M., Paul Aoun, Evelyn Perez, Ann Marie-Brun Zinkernagel, James W. Simpkins and Neeraj Agarwal, Role of Nonfeminizing Estrogen Analogues in Neuroprotection of Rat Retinal Ganglion Cells against Glutamate-Induced Cytotoxicity, Free Radical Biol Med 38: 1152-1163, 2005.

Shao-Hua Yang, Ran Liu, Evelyn J. Perez, Xiaofei Wang, James W. Simpkins. Estrogens as protectants of the neurovacular unit against ischemic stroke, Current Drug Targets 4: 169-177, 2005.

Perez, E., R. Liu, S.H. Yang, Z.Y. Cai, D. F. Covey and James W. Simpkins, Neuroprotective effects of an estratriene analog are estrogen receptor independent in vitro and in vivo, Brain Research 1038:216-222, 2005.

Simpkins, James W. and R. Van Meter, Potential Testosterone Therapy for Hypogonadal Sexual Dysfunction in Women. Journal of Women’s Health 14: 428-430, 2005.
Jung, M., D. G. Watson, S. Jacobs, M. Rewal and J. W. Simpkins, Estrogen inhibits cellular distribution of protein kinase C€ in a binge model of ethanol dependence and withdrawal, European J Pharmacology 515: 62-72, 2005.
James W. Simpkins, Yi Wen, Evelyn Perez, ShaoHua Yang, and Xiaofei Wang, Role of Non-feminizing Estrogens in Brain Protection from Cerebral Ischemia: An Animal Model of Alzheimer’s Disease Neuropathology, New York Academy of Science 1052: 233-242, 2005.

Yi, K. D., J. C. Gwon, P.Pang and James W. Simpkins, Role of Protein Phosphatases in Estrogen-Mediated Neuroprotection, J. Neuroscience 25: 7191-7198, 2005.

Yi Wen, Shaohua Yang, Ran Liu, Saumyendra Sarkar and James W. Simpkins, Cell-cycle regulators are involved in transient cerebral ischemia induced neuronal apoptosis in female rats, FABS Letters 579: 4591-4599, 2005.

Rewal, M., Y. Wen, A Wilson, J.W. Simpkins, and M.E. Jung, Role of Paralbumin in estrogen protection from ethanol withdrawal syndromes, Alcoholism: Clinical and Experimental Research 10: 1837-1844, 2005.

Evelyn Perez, Zu Yun Cai, Douglas F. Covey, James W. Simpkins, Neuroprotective Effects of Estratriene Analogs: Structure-Activity Relationships and Molecular Optimization, Drug Development Research 68: 1-15, 2006.

Ran Liu, Yi Wen,  Evelyn Perez,  Xiaofei Wang,  Arthur L. Day, James W. Simpkins, Shao-Hua Yang, 17 β-Estradiol Attenuates Blood-Brain Barrier Disruption Through by Cerebral Ischemia-Reperfusion Injury in Female Rats, Brain Research 1060: 55-61, 2005.

Jung, M.E., D.G. Watson and J.W. Simpkins, Suppression of Protein Kinase Ce mediates 17b-estradiol-induced neuroprotection in an immortalized cell line J. Neurochemistry 745-755, 2005.

Anna Ratka, PhD, PharmD*, Vanessa Miller, MS, APRN, Dr.PH*, Kimberly Brown, RN*, Atulkumar Raut, MD*, Daisha Cipher, PhD#, and J.W. Simpkins,  Evaluation of the validity, reliability, and sensitivity of a Menopausal Vasomotor Symptoms (MVS) Survey in the Assessment of Hot Flashes, J Women’s Health 15: 77-89, 2006.

Singh, M., J.A. Dykens and J.W. Simpkins, Novel Mechanisms for Estrogen-Induced Neuroprotection, J Experimental Biology & Medicine 231: 514-521, 2006.
Wang, X, J. A. Dykens, E.J. Perz, R. Liu, S.-H. Yang, D.F. Covey and J.W. Simpkins, Neuroprotective effects of 17b-estradiol and non-feminizing estrogens against H2O2-induced toxicity in human neuroblastoma SK-N-SH cells, Molecular Pharmacology 70:395-404, 2006.

Yang, S.H., L. Prokai, and James W. Simpkins, Correspondence regarding Schwend and Gustafsson, " False positives in MALDI-TOF detection of ER beta in mitochondria" Biochemical and Biophysical Research Communications 345: 917-918, 2006.

Liu, R.,  S. H. Yang , J. Cutright, K.D. Yi , X Wang , Y-J Hu, A. L. Day, J. W. Simpkins,   Dose dependence and therapeutic window for the neuroprotective effects of 17 b-estradiol when administered after cerebral ischemia, Physiology & Behavior, In Press, 2006.

Yang, S.H., R. Liu, Y. Wen, A. M. Brun-Zinkernagel and J. W. Simpkins, Modulation of estrogen receptor alpha and beta after cerebral ischemia reperfusion injury in female rats, J Neurobiology, In Press, 2006.

Prokai, L., K. Prokai-Tatrai, P. Perjesi and J. W. Simpkins, Mechanistic insights into the direct antioxidant effects of estrogens, Drug Development Research, In Press, 2006.

Perez, E., X. Wang and J.W. Simpkins, Role of Antioxidant activity of estrogens in their potent neuroprotection, submitted Oxidative Stress and Neurological Disorders, Editor, G. Ali Queshi, Elsevier Publishing Company, Holland, In Press, 2006.

Cheryl D Bushnell, MD, MHS1, Patricia Hurn, PhD2, Carol Colton, PhD1, Virginia Miller, PhD3, Gregory del Zoppo, MD, MS4, Mitchell Elkind, MD5, Barney Stern, MD6, David Herrington, MD7, Gwendolyn Ford-Lynch, MD8, Philip Gorelick, MD, MPH9, Andra James, MD, MPH10, Candice Brown, PhD11, Emily Choi, MD12, Paul Bray, MD13, L Kristin Newby, MD, MHS14, Larry Goldstein, MD1 and James Simpkins, PhD15  Advancing the Study of Stroke in Women.   Summary and Recommendations for Future Research from an NINDS-Sponsored Multidisciplinary Working Group, Stroke, in Press, 2006.

Marianna E. Jung, Andrew M. Wilson, James W. Simpkins, A nonfeminizing estrogen analogue protects against ethanol withdrawal toxicity in immortalized hippocampal cells, J. Pharmacology and Experimental Therapeutics, In Press, 2006.

Wen Y, Yang S, Liu R, Perez E, Yi KD, Koulen P, Simpkins JW. 2004. Estrogen attenuates nuclear factor-kappa B activation induced by transient cerebral ischemia. 1008:147-154.

Yang SH; Liu R; Wen Y; Brun AM; Prokai L; Will Y; Dykens J; Koulen P; Simpkins JW. Mitochondrial localization of estrogen receptor beta. Proc. National Academy of Sciences USA , 101: 4130-4135, 2004.

Yang SH; Liu R; Wen Y; Brun AM; Prokai L; Will Y; Dykens J; Koulen P; Simpkins JW. 2004 Mitochondrial localization of estrogen receptor beta. Proc. National Academy of Sciences USA , 101: 4130-4135.

James W. Simpkins, Jian Wang, X. Wang, Evelyn Perez, Laszlo Prokai and James A. Dykens. 2005 Mitochondria Play a Central Role in Estrogen-Induced Neuroprotection, Current Drug Targets-CNS and Neurological Disorders, 4:69-83.

Prokai-Tatrai, K and Prokai L. 2005 Impact of metabolism on the safety of estrogen therapy. Ann. N Y. Acad Sci. 1052:243:57.

Yang, S.H., L. Prokai, and James W. Simpkins, Correspondence regarding Schwend and Gustafsson, " False positives in MALDI-TOF detection of ER beta in mitochondria" Biochemical and Biophysical Research Communications 345: 917-918, 2006.

Prokai, L., K. Prokai-Tatrai, P. Perjesi and J. W. Simpkins, Mechanistic insights into the direct antioxidant effects of estrogens, Drug Development Research, In Press, 2006.
           
Ratka A. 2005 Menopausal hot flashes and development of cognitive impairment. Ann NY Acad Sci  1052:11-26.
Ratka A, Miller V, Brown K, Raut A, Cipher D, Meczekalski B, Simpkins JW. 2006 Menopausal Vasomotor Symptoms (MVS) survey for assessment of hot flashes. J. Women’s Health (Larchmt) 15:77-89.

Cammarata PR, Chu S, Moor A, Wang Z, Yang SH, Simpkins JW. 2004 Subcellular distribution of native estrogen receptor alpha and beta subtypes in cultured human lens epithelial cells. Exp. Eye Res  78:861-71.

Moor AN, Gottipati S, Mallet RT, Sun J, Giblin FJ, Roque R, Cammarata PR. 2004 A putative mitochondrial mechanism for antioxidative cytoprotection by 17beta-estradiol. Exp. Eye Res. 78:933-44

Flynn JM and Cammarata PR. 2006. Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells. Mol Vis. 12:271-82.

Cammarata PR, Flynn J, Gottipati S, Chu S, Dimitrijevich S, Younes M, Skliris G, Murphy LC. 2005 Differential expression and comparative subcellular localization of estrogen receptor beta isoforms in virally transformed and normal cultured human lens epithelial cells. Exp Eye Res. 81: 165-75

Moor AN, Flynn JM, Gottipati S, Giblin FJ, Cammarata PR. 2005 17beta-estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress. Mitochondrion 5:235-47.

Clearfield M. 2004 Coronary heart disease risk reduction in postmenopausal women: the role of statin therapy and hormone replacement therapy. Prev Cardiol  7:131-6.

Kumar DM, Perez E, Cai ZY, Aoun P, Brun-Zinkernagel AM, Covey DF, Simpkins JW, Agarwal N. 2005 Role of nonfeminizing estrogen analogues in neuroprotection of rat retinal ganglion cells against glutamate-induced cytotoxicity. Free Radic Biol Med. 38:1152-63.